The immunological activity of a deletion mutant of influenza virus haemagglutinin lacking the globular region.
Identifieur interne : 001C00 ( Main/Exploration ); précédent : 001B99; suivant : 001C01The immunological activity of a deletion mutant of influenza virus haemagglutinin lacking the globular region.
Auteurs : H. Sagawa [Japon] ; A. Ohshima ; I. Kato ; Y. Okuno ; Y. IsegawaSource :
- The Journal of general virology [ 0022-1317 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN viral, Animaux, Données de séquences moléculaires, Délétion de séquence, Femelle, Glycoprotéine hémagglutinine du virus influenza, Grippe humaine (), Humains, Hémagglutinines virales (), Hémagglutinines virales (génétique), Hémagglutinines virales (immunologie), Lignée cellulaire, Modèles animaux de maladie humaine, Pliage des protéines, Souris, Souris de lignée BALB C, Séquence nucléotidique, Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins synthétiques (administration et posologie), Vaccins synthétiques (génétique), Vaccins synthétiques (immunologie), Virus de la grippe A (immunologie).
- MESH :
- administration et posologie : Vaccins antigrippaux, Vaccins synthétiques.
- génétique : Hémagglutinines virales, Vaccins antigrippaux, Vaccins synthétiques.
- immunologie : Hémagglutinines virales, Vaccins antigrippaux, Vaccins synthétiques, Virus de la grippe A.
- ADN viral, Animaux, Données de séquences moléculaires, Délétion de séquence, Femelle, Glycoprotéine hémagglutinine du virus influenza, Grippe humaine, Humains, Hémagglutinines virales, Lignée cellulaire, Modèles animaux de maladie humaine, Pliage des protéines, Souris, Souris de lignée BALB C, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, DNA, Viral, Disease Models, Animal, Female, Hemagglutinin Glycoproteins, Influenza Virus, Hemagglutinins, Viral (chemistry), Hemagglutinins, Viral (genetics), Hemagglutinins, Viral (immunology), Humans, Influenza A virus (immunology), Influenza Vaccines (administration & dosage), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Influenza, Human (prevention & control), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Protein Folding, Sequence Deletion, Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology).
- MESH :
- chemical , administration & dosage : Influenza Vaccines, Vaccines, Synthetic.
- chemical , chemistry : Hemagglutinins, Viral.
- chemical , genetics : Hemagglutinins, Viral, Influenza Vaccines, Vaccines, Synthetic.
- chemical , immunology : Hemagglutinins, Viral, Influenza Vaccines, Vaccines, Synthetic.
- chemical : DNA, Viral, Hemagglutinin Glycoproteins, Influenza Virus.
- immunology : Influenza A virus.
- prevention & control : Influenza, Human.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Disease Models, Animal, Female, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Protein Folding, Sequence Deletion.
Abstract
A deletion mutant of influenza virus haemagglutinin (HA; headless HA) lacking the globular region was expressed in CV-1 cells and detected with a monoclonal antibody, C179, which recognizes a conformational epitope in the middle of the stem region of HA and neutralizes all H1 and H2 subtypes. The cDNA coding for the headless HA was constructed from influenza virus A/Okuda/57 (H2N2), which was also used to select C179. The conformational epitope recognized by C179 was highly stable even after removal of the globular region. The survival rate of mice immunized with the headless HA and challenged with lethal influenza virus A/FM/l/47 (H1N1) was significantly higher than that of the control mice. The headless HA has the potential to induce cross-protection against influenza virus infection.
DOI: 10.1099/0022-1317-77-7-1483
PubMed: 8757990
Affiliations:
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Le document en format XML
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headless HA) lacking the globular region was expressed in CV-1 cells and detected with a monoclonal antibody, C179, which recognizes a conformational epitope in the middle of the stem region of HA and neutralizes all H1 and H2 subtypes. The cDNA coding for the headless HA was constructed from influenza virus A/Okuda/57 (H2N2), which was also used to select C179. The conformational epitope recognized by C179 was highly stable even after removal of the globular region. The survival rate of mice immunized with the headless HA and challenged with lethal influenza virus A/FM/l/47 (H1N1) was significantly higher than that of the control mice. The headless HA has the potential to induce cross-protection against influenza virus infection.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Isegawa, Y" sort="Isegawa, Y" uniqKey="Isegawa Y" first="Y" last="Isegawa">Y. Isegawa</name><name sortKey="Kato, I" sort="Kato, I" uniqKey="Kato I" first="I" last="Kato">I. Kato</name><name sortKey="Ohshima, A" sort="Ohshima, A" uniqKey="Ohshima A" first="A" last="Ohshima">A. Ohshima</name><name sortKey="Okuno, Y" sort="Okuno, Y" uniqKey="Okuno Y" first="Y" last="Okuno">Y. Okuno</name></noCountry><country name="Japon"><noRegion><name sortKey="Sagawa, H" sort="Sagawa, H" uniqKey="Sagawa H" first="H" last="Sagawa">H. Sagawa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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